8ZLG
Crystal structure of a beta-1,4-endoglucanase from Bispora sp. MEY-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-12 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 115.101, 115.101, 107.712 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.440 - 2.250 |
| R-factor | 0.1865 |
| Rwork | 0.183 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.100 |
| High resolution limit [Å] | 2.250 | 2.340 |
| Rmeas | 0.147 | |
| Rpim | 0.073 | 0.073 |
| Number of reflections | 26711 | 16508 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 80.0 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.961 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Tris, pH 7.5, 0.2 M sodium acetate, 32% PEG 4000 |
