8ZKC

iron-sulfur cluster transfer protein ApbC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 2 2
Unit cell lengths	74.270, 74.270, 167.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.510 - 2.850
R-factor	0.2371
Rwork	0.235
R-free	0.27910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.478
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.510	2.910
High resolution limit [Å]	2.850	2.850
Rmerge	0.126
Rmeas	0.129
Rpim	0.026	0.706
Number of reflections	11707	845
<I/σ(I)>	21.1	1.2
Completeness [%]	99.9
Redundancy	24.3
CC(1/2)	1.000	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	tri-potassium citrate, PEG 3350