8ZI6

Crystal structure of SrUGT76G4 in complex with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 31
Unit cell lengths	111.780, 111.780, 91.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.990 - 2.800
R-factor	0.27244
Rwork	0.268
R-free	0.35122
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.325
Data reduction software	MOSFLM (7.4.0)
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.990	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.248	0.912
Rmeas	0.267	1.003
Rpim	0.094	0.397
Total number of observations	209055	20469
Number of reflections	30343	4019
<I/σ(I)>	5.4	2.1
Completeness [%]	96.1
Redundancy	6.9	5.1
CC(1/2)	0.971	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	293	0.2 M NaSCN, 0.1 M NaCitrate pH5.5, 10-35% PEG3350, 8% D-Sorbitol