8ZI5

Crystal structure of a beta-1,4-endoglucanase from Bispora sp. MEY-1 in complex with mannotetrose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	69.721, 88.593, 175.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.510 - 1.730
R-factor	0.1531
Rwork	0.151
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.994
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.510	50.000	1.760
High resolution limit [Å]	1.730	4.690	1.730
Rmerge	0.117	0.064	0.403
Rmeas	0.123	0.069	0.429
Rpim	0.039	0.025	0.144
Number of reflections	110885	5731	5590
<I/σ(I)>	6.2
Completeness [%]	97.6	94.5	100
Redundancy	10.5	9.3	8.7
CC(1/2)	0.964	0.969	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M Tris, pH 7.5, 0.2 M sodium acetate, 32% PEG 4000