8ZHS

Structure of Mbp-Bte1 fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	104.175, 253.874, 56.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.130 - 2.400
R-factor	0.1895
Rwork	0.188
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.173
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.130	2.500
High resolution limit [Å]	2.400	2.400
Number of reflections	55319	4190
<I/σ(I)>	1.56
Completeness [%]	93.2
Redundancy	10.3
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	10% v/v 2-Propanol, 0.1 M BICINE pH 8.5 and 30% w/v PEG1500