8ZH5
The Crystal Structure of the ROCK1 from Biortus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-07 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 166.283, 166.283, 64.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.161 - 3.700 |
Rwork | 0.208 |
R-free | 0.25950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.790 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.730 | 4.050 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.235 | 0.917 |
Number of reflections | 9205 | 2184 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 91.4 | |
Redundancy | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MgAC2, 0.1M NaAC pH4.5, 8% PEG 8000, 1.0M NDSB-256 |