8ZGD
The Crystal Structure of the NLRP1_LRR domain from Biortus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-27 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.952989 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 84.582, 84.582, 132.495 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.130 - 2.050 |
| Rwork | 0.176 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.929 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.130 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.210 | 0.964 |
| Number of reflections | 18288 | 1387 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20mM MgCl2, 0.1M Tris pH 8.0, 19% polyacrylic acid 5100 |
