8ZF8

L-methionine oxidase from Burkholderia bacterium, K304A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	122.031, 122.031, 295.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.790 - 2.100
R-factor	0.20941
Rwork	0.208
R-free	0.23282
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.970
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	62033	123293
<I/σ(I)>	26.4
Completeness [%]	99.9
Redundancy	26.6
CC(1/2)	0.999	0.953

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	25%(v/v)1,4-Butandiol, 100 mM Tris-HCl (pH 8.0)