8ZEX
Biosensor HaloKbp1a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.590, 50.280, 58.830 |
| Unit cell angles | 94.00, 101.32, 99.97 |
Refinement procedure
| Resolution | 18.240 - 1.660 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.20410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.043 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.240 | 1.720 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 55656 | 5509 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.997 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M PCTP, pH 6.0 25% PEG1500 |
