8ZD3
Crystal structure of ALPK1-N+K in complex with CDP-heptose
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.787, 65.944, 108.679 |
| Unit cell angles | 89.62, 107.77, 111.12 |
Refinement procedure
| Resolution | 30.550 - 2.300 |
| R-factor | 0.2407 |
| Rwork | 0.240 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.782 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.550 | 2.386 |
| High resolution limit [Å] | 2.300 | 2.303 |
| Rmerge | 0.041 | 0.354 |
| Number of reflections | 124422 | 9482 |
| <I/σ(I)> | 12.99 | |
| Completeness [%] | 96.4 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 8% tacsimate ph 6.0, 14% PEG 3350 |
