8ZC7
Crystallographic analysis of MitM, which catalyzes the post-mitosane modification in mitomycin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2022-12-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97891 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.357, 113.357, 110.044 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.366 - 2.660 |
| R-factor | 0.2042 |
| Rwork | 0.200 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.360 | 2.290 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Number of reflections | 23376 | 23376 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.2 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.998 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.2 M Sodium nitrate, 0.1 M Bis-Tris propane, PH 6.5, 20 % w/v PEG 3350 |
