8ZB5
Crystal structure of NudC from Mycobacterium abscessus in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 131.999, 131.999, 174.314 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.980 - 2.600 |
| R-factor | 0.22282 |
| Rwork | 0.220 |
| R-free | 0.26882 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.265 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.980 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.080 | 1.020 |
| Rmeas | 0.084 | 1.079 |
| Rpim | 0.024 | 0.343 |
| Total number of observations | 323086 | 29027 |
| Number of reflections | 26851 | 3038 |
| <I/σ(I)> | 17.5 | 2.5 |
| Completeness [%] | 95.1 | |
| Redundancy | 12 | 9.6 |
| CC(1/2) | 0.999 | 0.705 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% PEG8000, 0.1 M imidazole pH 8.0, 0.2 M Ca(OAc)2 |
