8ZB3
Crystal structure of NudC from Mycobacterium abscessus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.009, 133.549, 118.730 |
| Unit cell angles | 90.00, 95.22, 90.00 |
Refinement procedure
| Resolution | 39.820 - 2.200 |
| R-factor | 0.19845 |
| Rwork | 0.197 |
| R-free | 0.22931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.349 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.820 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.098 | 0.925 |
| Rmeas | 0.107 | 1.010 |
| Rpim | 0.042 | 0.397 |
| Total number of observations | 534605 | 29284 |
| Number of reflections | 84252 | 4455 |
| <I/σ(I)> | 9.4 | 2.3 |
| Completeness [%] | 99.2 | |
| Redundancy | 6.3 | 6.6 |
| CC(1/2) | 0.994 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 10% PEG8000, 0.1 M imidazole pH 8.0, 0.2 M Ca(OAc)2 |
