8ZAZ

Crystal structure of Chitinase 3-like protein 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	109.480, 122.390, 137.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.970 - 2.310
Rwork	0.201
R-free	0.25680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.688
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.840	2.370
High resolution limit [Å]	2.310	2.310
Rmerge	0.167	1.844
Number of reflections	81373	5941
<I/σ(I)>	12.2
Completeness [%]	100.0
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	10% PEG8000, 0.5 M NaCl, 0.1 M sodium citrate pH 3.5