8Z9K

Crystal structure of LINE-1 ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 61 2 2
Unit cell lengths	93.247, 93.247, 262.655
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.670 - 3.860
R-factor	0.3151
Rwork	0.314
R-free	0.34570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.005
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.750	4.060
High resolution limit [Å]	3.860	3.860
Number of reflections	6885	863
<I/σ(I)>	22
Completeness [%]	98.2
Redundancy	18
CC(1/2)	0.999	0.976

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.02 M Calcium Chloride dihydrate 0.1 M Sodium Acetate trihydrate pH 4.6 30% v/v 2-Methyl-2,4-pentanediol