8Z9I

Crystal structure of RaTG13 RBD bound to Rhinolophus affinis ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	100.925, 273.391, 165.103
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.653 - 3.011
R-factor	0.1962
Rwork	0.194
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.330
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.119
High resolution limit [Å]	3.011	3.011
Number of reflections	44473	3985
<I/σ(I)>	8.2
Completeness [%]	98.7
Redundancy	12
CC(1/2)	0.990	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M Sodium citrate tribasic dihydrate pH 5.0,30% v/v Jeffamine ED-2001 pH 7.0