8Z9F
Crystal structure of glyoxylate reductase from Acetobacter aceti in complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.598, 192.500, 118.213 |
| Unit cell angles | 90.00, 95.07, 90.00 |
Refinement procedure
| Resolution | 48.280 - 1.600 |
| R-factor | 0.2124 |
| Rwork | 0.211 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.958 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.280 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.122 | 1.181 |
| Rpim | 0.049 | 0.540 |
| Number of reflections | 321671 | 26268 |
| <I/σ(I)> | 17.35 | 1.62 |
| Completeness [%] | 95.6 | 78.46 |
| Redundancy | 6.9 | 5.6 |
| CC(1/2) | 0.999 | 0.588 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.4M MES, 0.2M CaCl2, 15% PEG 6000 |
