8Z96
Crystal structure of CrtAgo/TIR-APAZ in complex with guide DNA and 21-nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.94138 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 133.574, 133.574, 167.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.680 - 3.360 |
| R-factor | 0.2489 |
| Rwork | 0.247 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.753 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.360 | 3.360 |
| Rpim | 0.052 | 0.276 |
| Number of reflections | 25189 | 1220 |
| <I/σ(I)> | 14.8 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.3 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2% v/v Tacsimate, pH 7.0, 5% v/v 2-Propanol, 0.1 M Imidazole, pH 7.0, 8% w/v Polyethylene glycol 3350 |
