8Z8V
Crystal structure of human serum albumin in complex with ALB8(VHH) domain of ozoralizumab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 60.290, 60.900, 62.810 |
Unit cell angles | 89.51, 63.72, 76.49 |
Refinement procedure
Resolution | 45.300 - 2.050 |
R-factor | 0.20432 |
Rwork | 0.202 |
R-free | 0.23854 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.518 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.300 | 2.170 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 47566 | 7440 |
<I/σ(I)> | 7.09 | |
Completeness [%] | 97.6 | |
Redundancy | 3.9 | |
CC(1/2) | 0.994 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.7 | 293 | 100 mM SPG Buffer (pH4.7) 21 % PEG1500 |