8Z8Q
Crystal structure of 2'-dG riboswitch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.730, 51.275, 52.484 |
| Unit cell angles | 113.83, 114.99, 90.05 |
Refinement procedure
| Resolution | 27.340 - 3.010 |
| R-factor | 0.3177 |
| Rwork | 0.317 |
| R-free | 0.32710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.734 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.610 | 3.090 |
| High resolution limit [Å] | 3.010 | 3.010 |
| Number of reflections | 7298 | 538 |
| <I/σ(I)> | 2.9 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.957 | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.04 M Lithium chloride, 0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate 0.04 M Sodium cacodylate trihydrate pH 7.0 30% v/v (+/-)-2-Methyl-2,4-pentanediol 0.012 M Spermine tetrahydrochloride |
