8Z62
Crystal structure of rRNA (uracil-C5)-methyltransferase from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2023-04-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.890, 96.610, 122.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.830 - 3.200 |
| R-factor | 0.17247 |
| Rwork | 0.169 |
| R-free | 0.23723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF-O58994-F1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.407 |
| Data reduction software | MOSFLM (7.4.0) |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.610 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.224 | 0.536 |
| Rmeas | 0.239 | 0.572 |
| Rpim | 0.083 | 0.198 |
| Total number of observations | 149497 | 26915 |
| Number of reflections | 18612 | 3314 |
| <I/σ(I)> | 8.3 | 3.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 8 | 8.1 |
| CC(1/2) | 0.986 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 0.1 M HEPES sodium pH 7.5, 2% (v/v) PEG 400, 2.0 M ammounium sulfate |
