8Z5B

The X-Ray crystal structure of multicopper oxidase from Bacillus freudenreichii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-18
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9785
Spacegroup name	P 41 21 2
Unit cell lengths	74.590, 74.590, 230.946
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.020 - 1.400
R-factor	0.1587
Rwork	0.158
R-free	0.17310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.020	1.450
High resolution limit [Å]	1.400	1.400
Rmeas		0.030
Number of reflections	124244	124244
<I/σ(I)>	24.2
Completeness [%]	96.3
Redundancy	14.3
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	289.15	0.1 M Tris pH 8.5, 20% v/v Ethanol