8Z59

The X-Ray crystal structure of multicopper oxidase from Sulfurimonas sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9785
Spacegroup name	P 1
Unit cell lengths	86.636, 103.389, 131.906
Unit cell angles	104.86, 103.96, 98.35

Refinement procedure

Resolution	39.520 - 2.580
R-factor	0.1921
Rwork	0.191
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fqg
RMSD bond length	0.010
RMSD bond angle	1.348
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.580	2.580
Rmeas		14.900
Number of reflections	126901	126901
<I/σ(I)>	7.4
Completeness [%]	96.5
Redundancy	3.4
CC(1/2)	0.984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		290.15	1% w/v Tryptone, 0.001 M Sodium azide, 0.05 M HEPES sodium pH 7.0, 12% w/v Polyethylene glycol 3,350