8Z55

Crystal structure of human SIRT5 in complex with succPrx1 and ADP ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	46.198, 73.345, 163.938
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.090 - 1.830
R-factor	0.2135
Rwork	0.212
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.896
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.980	1.870
High resolution limit [Å]	1.830	1.830
Rmeas	0.070	1.110
Number of reflections	25082	1533
<I/σ(I)>	19	2.3
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.999	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% PEG 3350, 0.1M Tris-HCl pH 8.5