8Z51

Hen Egg-White Lysozyme (HEWL) complexed with Emetine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-14
Detector	Bruker PHOTON III
Wavelength(s)	1.5
Spacegroup name	P 43 21 2
Unit cell lengths	79.191, 79.191, 35.335
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.960 - 1.500
R-factor	0.178
Rwork	0.175
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.257
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.960	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.100	0.671
Rmeas	0.104	0.731
Rpim	0.025	0.287
Number of reflections	18512	3447
<I/σ(I)>	17.2	2.1
Completeness [%]	99.8	99.8
Redundancy	13	6.2
CC(1/2)	0.999	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Na-acetate pH 4.5, 6.5 %(w/v) NaCl