8Z4I
crystal structure of Cdn1 in complex with cA3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 193.15 |
| Detector technology | PIXEL |
| Collection date | 2024-03-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979540 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.490, 61.300, 103.570 |
| Unit cell angles | 90.00, 97.55, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.800 |
| R-factor | 0.2506 |
| Rwork | 0.248 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.585 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.330 | 2.860 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.132 | 1.803 |
| Rmeas | 0.143 | 1.952 |
| Rpim | 0.055 | 0.742 |
| Total number of observations | 157954 | 11888 |
| Number of reflections | 23893 | 1748 |
| <I/σ(I)> | 12.3 | 1.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.6 | 6.8 |
| CC(1/2) | 0.998 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 30% PEG 400, 0.1M Calcium acetate hydrate, 0.1M MES(pH6.5) |
