8Z4B

Crystal structure of LysB22-AspB28 insulin analog at ambient structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	293
Detector technology	PIXEL
Collection date	2022-02-17
Detector	RIGAKU HyPix-Arc 150
Wavelength(s)	1.5
Spacegroup name	H 3
Unit cell lengths	80.376, 80.376, 38.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.350 - 2.500
R-factor	0.3
Rwork	0.300
R-free	0.35680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.670
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.640	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	3104	3104
<I/σ(I)>	6.88
Completeness [%]	97.7
Redundancy	20
CC(1/2)	0.952	0.358

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.4 M NaCl, 100 mM Tris-HCl at pH 7.4, 6 mM ZnCI2, 20 (w/v) poly(ethylene glycol) PEG-8000