8Z3D
SFX structure of CraCRY 1 us after photoexcitation of the oxidized protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2021-04-25 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.840, 65.600, 153.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.580 - 2.000 |
| R-factor | 0.2006 |
| Rwork | 0.200 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fn3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.603 |
| Data reduction software | CrystFEL |
| Data scaling software | pointless |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.070 | 1.910 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Number of reflections | 43294 | 2113 |
| <I/σ(I)> | 6.76 | 1.42 |
| Completeness [%] | 100.0 | |
| Redundancy | 275 | |
| CC(1/2) | 0.991 | 0.515 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 277 | 0.1 M MES, pH 5.6 (w/v) PEG 4000 |
