8Z3B
Crystal structure of GSTd2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-01-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.124, 82.355, 106.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.355 - 2.300 |
Rwork | 0.185 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.934 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_360) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.355 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 23353 | 2288 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 99.8 | |
Redundancy | 7.2 | |
CC(1/2) | 0.840 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 15% PEG3350, and 0.12M Tris at a pH of 7.0 |