8Z2W
Crystal structure of apo- exo-beta-(1,3)-glucanase from Aspergillus oryzae (AoBgl)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-09-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.488, 81.488, 115.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.570 - 1.750 |
| R-factor | 0.14533 |
| Rwork | 0.144 |
| R-free | 0.17175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.617 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.570 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.090 | |
| Number of reflections | 45212 | 2452 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.1 | |
| CC(1/2) | 1.000 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 1.6M sodium citrate tribasic dihydrate |
