8Z2B

Crystal structure of apo Aspergillus terreus glutamate dehydrogenase (AtGDH) in the partially closed conformation (form II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-08-17
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32
Unit cell lengths	101.690, 101.690, 271.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.890 - 2.850
Rwork	0.192
R-free	0.26770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xvi
RMSD bond length	0.007
RMSD bond angle	1.654
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	73339	7224
<I/σ(I)>	9.3
Completeness [%]	99.9
Redundancy	5.65
CC(1/2)	0.980	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2 M MgCl2, 0.1 M Tris pH 7, and 10% PEG 8000