8Z2A

Crystal structure of Aspergillus terreus glutamate dehydrogenase (AtGDH) with sequential mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	100
Detector technology	CCD
Collection date	2024-02-26
Detector	RAYONIX MX-225
Wavelength(s)	0.9794
Spacegroup name	P 21 3
Unit cell lengths	143.590, 143.590, 143.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 3.100
R-factor	0.2038
Rwork	0.200
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ecr
RMSD bond length	0.013
RMSD bond angle	1.363
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.300
High resolution limit [Å]	3.100	3.100
Number of reflections	18106	3057
<I/σ(I)>	14.2
Completeness [%]	99.5
Redundancy	20
CC(1/2)	0.990	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.2 M Sodium thiocyanate, 20% PEG 3350