8Z28

FK506-binding protein 1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	291.15
Detector technology	PIXEL
Collection date	2023-04-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	55.453, 61.066, 68.072
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.040 - 1.350
R-factor	0.1589
Rwork	0.158
R-free	0.18460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.897
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.040	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.052	0.294
Rmeas	0.055	0.321
Rpim	0.016	0.127
Number of reflections	51043	4989
<I/σ(I)>	26.02	4.58
Completeness [%]	99.2
Redundancy	11.7
CC(1/2)	0.999	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	4%v/v TacsimateTM pH 8.0,12% w/v Polyethylene glycol3,350