8Z1J
SFX structure of dark adapted CraCRY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL2 |
| Synchrotron site | SACLA |
| Beamline | BL2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2021-05-25 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.840, 65.600, 153.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.060 - 1.640 |
| R-factor | 0.1404 |
| Rwork | 0.140 |
| R-free | 0.15730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fn3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.999 |
| Data reduction software | CrystFEL |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.090 | 1.830 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Number of reflections | 63708 | 3083 |
| <I/σ(I)> | 9.505 | 1.37 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 909.6 | 110.5 |
| CC(1/2) | 0.997 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 5.6 | 277 | 50 mM sodium phosphate, pH 7.8, 250 mM NaCl, 20% glycerol |
