8Z0X
Crystal structure of glyoxylate reductase from Acetobacter aceti in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-04 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.454, 191.880, 117.846 |
| Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
| Resolution | 48.180 - 1.600 |
| R-factor | 0.2025 |
| Rwork | 0.202 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.180 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.175 | 0.655 |
| Rmeas | 0.190 | 0.743 |
| Rpim | 0.072 | 0.340 |
| Number of reflections | 330996 | 32100 |
| <I/σ(I)> | 25.63 | 7.17 |
| Completeness [%] | 99.4 | 96.54 |
| Redundancy | 6.5 | 4.6 |
| CC(1/2) | 0.997 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.4M MES, 0.2M CaCl2, 15% PEG 6000 |
