8Z0E

Structure of human Glutamate oxaloacetate transaminase 1 (GOT1) mutant - P15R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	89.952, 97.072, 97.427
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.835 - 1.820
R-factor	0.1701
Rwork	0.168
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.802
Data reduction software	DIALS
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.835	1.820
High resolution limit [Å]	1.820	1.820
Rmerge		0.570
Number of reflections	75962	11067
<I/σ(I)>	15.4
Completeness [%]	98.8
Redundancy	4.8	3.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	289	0.1M Bis Tris pH:6.4,22% PEG5000MME