8Z0C

Crystal structure of a TetR family regulator AmvR from Acinetobacter Baumannii with spermidine bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	59.840, 65.520, 100.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.920 - 2.550
R-factor	0.2302
Rwork	0.226
R-free	0.31630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.420	2.641
High resolution limit [Å]	1.460	1.460
Number of reflections	13420	1296
<I/σ(I)>	9.1
Completeness [%]	99.5
Redundancy	3.7
CC(1/2)	0.997	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.02 M Sodium potassium phosphate pH 8.5 0.1 M Bis Tris Propane pH 8.5 20 % w/v PEG 3350 10 % v/v Ethylene glycol