8YZY
STC SydA mutant - W297Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-26 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.892, 127.635, 89.143 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 27.300 - 1.730 |
| R-factor | 0.1559 |
| Rwork | 0.155 |
| R-free | 0.19200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 8.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.300 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rpim | 0.046 | 0.046 |
| Number of reflections | 114819 | 114786 |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 94.2 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.1M Ammonium tartrate dibasic pH 7.0, 12% w/v Polyethylene glycol 3350 |
