8YZU
STC truncated SydA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-11 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.151, 125.000, 93.140 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 31.250 - 2.010 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.914 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.250 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmeas | 0.174 | |
| Rpim | 0.068 | 0.068 |
| Number of reflections | 79134 | 79088 |
| <I/σ(I)> | 8.06 | |
| Completeness [%] | 92.6 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.2M ammonium tartrate dibasic, 20% w/v polyethylene glycol 3350, pH 7.0 |
