8YZN

Crystal structural analysis of PaL

Replaces:  8JJ7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 2
Unit cell lengths	81.774, 104.782, 62.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.164 - 1.799
Rwork	0.144
R-free	0.17570
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.511
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.164	1.840
High resolution limit [Å]	1.799	1.800
Rmerge	0.080	0.470
Number of reflections	48967	49315
<I/σ(I)>	23.8
Completeness [%]	97.0
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Disodium malonate 0.1 M, pH7.0 , PEG3350 18%