8YXW

TRIP4 ASCH domain in complex with a 12bp dsDNA (5'-TGAGGTACCTCC-3')

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	66.597, 66.597, 199.648
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.830 - 2.100
R-factor	0.1965
Rwork	0.194
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.975
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.149	0.090	0.806
Rmeas	0.154	0.094	0.837
Rpim	0.039	0.024	0.224
Total number of observations	456895
Number of reflections	29191	1493	1440
<I/σ(I)>	3
Completeness [%]	100.0	99.5	100
Redundancy	15.7	15.2	14.3
CC(1/2)	0.997	0.995	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2 M Ammonium acetate 0.1 M BIS-TRIS pH 5.5 25% w/v Polyethylene glycol 3,350