8YXU

Crystal structure of CsoS1A/B (modeled with CsoS1A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-05
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9791
Spacegroup name	P 63
Unit cell lengths	67.310, 67.310, 64.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.655 - 2.100
R-factor	0.2197
Rwork	0.216
R-free	0.25250
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.900
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.290	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.143	0.482
Rmeas	0.147	0.495
Rpim	0.033	0.108
Number of reflections	9780	801
<I/σ(I)>	12.8
Completeness [%]	99.9	100
Redundancy	18.1
CC(1/2)		0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Lithium citrate tribasic tetrahydrate and 20% (w/v) polyethylene glycol (PEG) 3350