8YXN
X-ray structure of Clostridium perfringens autolysin catalytic domain in the P1 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-10-19 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 37.130, 47.520, 64.310 |
Unit cell angles | 94.79, 89.92, 96.29 |
Refinement procedure
Resolution | 36.503 - 1.600 |
Rwork | 0.163 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.634 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.530 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 56867 | 4154 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 98.9 | |
Redundancy | 5.6 | |
CC(1/2) | 0.995 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% (w/v) PEG 3000, 100mM CHES/ sodium hydroxide |