8YXB
Crystal structure of the HSA complex with ceftriaxone and myristate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-05 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 38.893, 93.786, 94.747 |
Unit cell angles | 105.73, 100.03, 89.71 |
Refinement procedure
Resolution | 38.260 - 2.200 |
R-factor | 0.2346 |
Rwork | 0.233 |
R-free | 0.26420 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.001 |
RMSD bond angle | 0.386 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 2.330 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.055 | 0.714 |
Number of reflections | 63358 | 10070 |
<I/σ(I)> | 17.76 | 2.32 |
Completeness [%] | 98.1 | 96.5 |
Redundancy | 7.2 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 32% PEG 3350, 50mM potassium phosphate pH 7.0 |