8YXA
Crystal structure of the HSA complex with cefazolin and myristate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-05 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 |
Unit cell lengths | 38.801, 94.351, 94.461 |
Unit cell angles | 105.19, 101.78, 90.03 |
Refinement procedure
Resolution | 37.900 - 2.500 |
R-factor | 0.2701 |
Rwork | 0.268 |
R-free | 0.31890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.476 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.600 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.048 | 0.694 |
Number of reflections | 42775 | 6786 |
<I/σ(I)> | 11.1 | 1.22 |
Completeness [%] | 99.9 | 96.4 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 32% PEG 3350, 50mM potassium phosphate pH 7.0 |