8YWP

Crystal structure of the Fab fragment of anti-IL-6 antibody I9H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	APEX II CCD
Wavelength(s)	1.542
Spacegroup name	P 62 2 2
Unit cell lengths	126.360, 126.360, 143.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.790 - 2.500
R-factor	0.2603
Rwork	0.258
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yk4
RMSD bond length	0.002
RMSD bond angle	0.587
Data reduction software	XDS (20190315)
Data scaling software	XSCALE (20190315)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18_3855)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.560
High resolution limit [Å]	2.500	2.500
Rpim	0.060	1.766
Number of reflections	23296	1423
<I/σ(I)>	10.7	0.4
Completeness [%]	97.1	84.7
Redundancy	23.8	2.3
CC(1/2)	0.990	0.131

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM Tris-HCl, 20% PEG 20000