8YWD
Crystal structure of trehalose synthase mutant N253C from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.321, 196.852, 131.188 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 22.330 - 2.650 |
| R-factor | 0.1753 |
| Rwork | 0.172 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.063 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 137854 | 43914 |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.998 | 0.584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Tris-HCl pH 8.5, 8% (w/v) polyethylene glycol (PEG) 8000 |
