8YVH
NADPH complex of Imine Reductase Mutant(M6) from Pochonia chlamydosporia 170
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-25 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.398, 117.950, 63.529 |
| Unit cell angles | 90.00, 100.89, 90.00 |
Refinement procedure
| Resolution | 35.090 - 2.100 |
| R-factor | 0.17061 |
| Rwork | 0.167 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.580 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.090 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.166 |
| Number of reflections | 36140 | 1528 |
| <I/σ(I)> | 13.36 | 2.08 |
| Completeness [%] | 98.4 | 84.2 |
| Redundancy | 3.99 | 2.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | PEG3350, magnesium chloride, Tris-Hcl pH 8.5 |
