8YVB

Crystal structure of Caenorhabditis elegans ZIM-1 ZF1-2-CTD domain in complex with Chromosome II/III pairing center

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	C 2 2 21
Unit cell lengths	55.767, 62.464, 101.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 1.900
R-factor	0.192
Rwork	0.190
R-free	0.23890
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.585
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.930
High resolution limit [Å]	1.900	5.130	1.900
Rmerge	0.097	0.061	0.972
Rmeas	0.101	0.064	1.015
Rpim	0.028	0.018	0.289
Total number of observations	183804
Number of reflections	14363	787	696
<I/σ(I)>	6.5
Completeness [%]	99.9	99.4	100
Redundancy	12.8	12.1	11.9
CC(1/2)	0.997	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% w/v PEG 600 and 0.1 M HEPES, pH 7.4