8YVA

Crystal structure of Caenorhabditis elegans ZIM-2 ZF1-2-CTD domain in complex with Chromosome V pairing center

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	36.019, 46.351, 59.228
Unit cell angles	79.41, 74.33, 72.86

Refinement procedure

Resolution	32.990 - 1.800
R-factor	0.1781
Rwork	0.176
R-free	0.22050
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.059	0.039	0.343
Rmeas	0.070	0.046	0.413
Rpim	0.037	0.024	0.228
Total number of observations	108350
Number of reflections	31655	1617	1434
<I/σ(I)>	7.5
Completeness [%]	96.9	98.5	89.5
Redundancy	3.4	3.6	2.9
CC(1/2)	0.989	0.996	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% w/v PEG 4000 and 0.2 M Imidazole malate pH 6.0